The uridine in “U-turn”: Contributions to tRNA-ribosomal binding
نویسندگان
چکیده
منابع مشابه
Contribution of ribosomal residues to P-site tRNA binding
Structural studies have revealed multiple contacts between the ribosomal P site and tRNA, but how these contacts contribute to P-tRNA binding remains unclear. In this study, the effects of ribosomal mutations on the dissociation rate (k(off)) of various tRNAs from the P site were measured. Mutation of the 30S P site destabilized tRNAs to various degrees, depending on the mutation and the specie...
متن کاملRole of hybrid tRNA-binding states in ribosomal translocation.
During translation, tRNAs must move rapidly to their adjacent sites in the ribosome while maintaining precise pairing with mRNA. This movement (translocation) occurs in a stepwise manner with hybrid-state intermediates, but it is unclear how these hybrid states relate to kinetically defined events of translocation. Here we analyze mutations at position 2394 of 23S rRNA in a pre-steady-state kin...
متن کاملOn the Phe-tRNA induced binding of fluorescent oligonucleotides to the ribosomal decoding site.
Fluorescent oligonucleotides were prepared by dansylation of 5'-amino uridylates of varying chainlength. Except for the trinucleoside diphosphate, they stimulated the binding of PhetRNA TO 70S E. coli ribosomes as efficiently as underivatised oligouridylic acids of comparable chainlength. The ternary ribosomal complex [70S X Phe-tRNA X dansyl-n5'U(pU)4] was separated from excess oligonucleotide...
متن کاملEnergy U-turn in Germany
Following the disaster at Fukushima-Daiichi, chancellor Angela Merkel decided to speed up rather than slow down Germany's exit from nuclear power.
متن کاملAutoantibodies make a U-turn
Like the immune response itself, our efforts to understand the "rules" for self-nonself discrimination are constantly evolving. The discovery of pattern recognition receptors-the Toll-like receptor (TLR) family in particular-shifted the emphasis of self-nonself recognition from lymphocytes functioning in the adaptive immune system to antigen-presenting cells (APCs) functioning in the innate imm...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: RNA
سال: 1999
ISSN: 1355-8382
DOI: 10.1017/s1355838299981931